Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species
Identifieur interne : 008776 ( Main/Exploration ); précédent : 008775; suivant : 008777Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species
Auteurs : S. Epis [Italie] ; D. Sassera [Italie] ; T. Beninati [Australie] ; N. Lo [Australie] ; L. Beati [États-Unis] ; J. Piesman [États-Unis] ; L. Rinaldi [Italie] ; K. D. Mccoy [France] ; A. Torina [Italie] ; L. Sacchi [Italie] ; E. Clementi [Italie] ; M. Genchi [Italie] ; S. Magnino [Italie] ; C. Bandi [Italie]Source :
- Parasitology [ 0031-1820 ] ; 2008.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
The hard tick Ixodes ricinus (Ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial bacterium, which has recently been named Midichloria mitochondrii. The objectives of this work were (i) to screen ixodid ticks for Midichloria-related bacteria and (ii) to determine whether these bacteria exploit the intra-mitochondrial niche in other tick species. Our main goal was to discover further models of this peculiar form of symbiosis. We have thus performed a PCR screening for Midichloria-related bacteria in samples of ixodid ticks collected in Italy, North America and Iceland. A total of 7 newly examined species from 5 genera were found positive for bacteria closely related to M. mitochondrii. Samples of the tick species Rhipicephalus bursa, found positive in the PCR screening, were analysed with transmission electron microscopy, which revealed the presence of bacteria both in the cytoplasm and in the mitochondria of the oocytes. There is thus evidence that bacteria invade mitochondria in at least 2 tick species. Phylogenetic analysis on the bacterial 16S rRNA gene sequences generated from positive specimens revealed that the bacteria form a monophyletic group within the order Rickettsiales. The phylogeny of Midichloria symbionts and related bacteria does not appear completely congruent with the phylogeny of the hosts.
Url:
DOI: 10.1017/S0031182007004052
Affiliations:
- Australie, France, Italie, États-Unis
- Colorado, Géorgie (États-Unis), Languedoc-Roussillon, Nouvelle-Galles du Sud, Occitanie (région administrative)
- Montpellier, Sydney
- Université de Sydney
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Le document en format XML
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Epis</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli Studi di Milano, Milano</wicri:regionArea><wicri:noRegion>Milano</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Sassera, D" sort="Sassera, D" uniqKey="Sassera D" first="D." last="Sassera">D. Sassera</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli Studi di Milano, Milano</wicri:regionArea><wicri:noRegion>Milano</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Beninati, T" sort="Beninati, T" uniqKey="Beninati T" first="T." last="Beninati">T. Beninati</name><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>School of Biological Sciences, The University of Sydney, New South Wales</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>§Present address: Faculty of Veterinary Science, The University of Sydney, New South Wales</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Lo, N" sort="Lo, N" uniqKey="Lo N" first="N." last="Lo">N. Lo</name><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>School of Biological Sciences, The University of Sydney, New South Wales</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Beati, L" sort="Beati, L" uniqKey="Beati L" first="L." last="Beati">L. Beati</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute of Arthropodology and Parasitology, Georgia Southern University, Statesboro, GA</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Piesman, J" sort="Piesman, J" uniqKey="Piesman J" first="J." last="Piesman">J. Piesman</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Vector Borne Infectious Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Rinaldi, L" sort="Rinaldi, L" uniqKey="Rinaldi L" first="L." last="Rinaldi">L. Rinaldi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Patologia e Sanità Animale, Universita degli Studi di Napoli Federico II, Napoli</wicri:regionArea><wicri:noRegion>Napoli</wicri:noRegion></affiliation></author><author><name sortKey="Mccoy, K D" sort="Mccoy, K D" uniqKey="Mccoy K" first="K. D." last="Mccoy">K. D. Mccoy</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Génétique et Evolution des Maladies Infectieuses, UMR CNRS-IRD 2724, IRD, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Torina, A" sort="Torina, A" uniqKey="Torina A" first="A." last="Torina">A. Torina</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Zooprofilattico Sperimentale della Sicilia, Palermo</wicri:regionArea><wicri:noRegion>Palermo</wicri:noRegion></affiliation></author><author><name sortKey="Sacchi, L" sort="Sacchi, L" uniqKey="Sacchi L" first="L." last="Sacchi">L. Sacchi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Biologia Animale, Università di Pavia, Piazza Botta 9, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Clementi, E" sort="Clementi, E" uniqKey="Clementi E" first="E." last="Clementi">E. Clementi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Biologia Animale, Università di Pavia, Piazza Botta 9, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Genchi, M" sort="Genchi, M" uniqKey="Genchi M" first="M." last="Genchi">M. Genchi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Biologia Animale, Università di Pavia, Piazza Botta 9, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Magnino, S" sort="Magnino, S" uniqKey="Magnino S" first="S." last="Magnino">S. Magnino</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Sezione di Pavia</wicri:regionArea><wicri:noRegion>Sezione di Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Bandi, C" sort="Bandi, C" uniqKey="Bandi C" first="C." last="Bandi">C. Bandi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli Studi di Milano, Milano</wicri:regionArea><wicri:noRegion>Milano</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Italie</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Parasitology</title><title level="j" type="abbrev">Parasitology</title><idno type="ISSN">0031-1820</idno><idno type="eISSN">1469-8161</idno><imprint><publisher>Cambridge University Press</publisher><pubPlace>Cambridge, UK</pubPlace><date type="published" when="2008">2008</date><biblScope unit="volume">135</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="485">485</biblScope><biblScope unit="page" to="494">494</biblScope></imprint><idno type="ISSN">0031-1820</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0031-1820</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ectoparasite</term><term>Ixodes ricinus</term><term>Ixodidae</term><term>Mitochondria</term><term>Parasite</term><term>Phylogeny</term><term>Polymerase chain reaction</term><term>Taxonomy</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Ectoparasite</term><term>Ixodes ricinus</term><term>Ixodidae</term><term>Mitochondrie</term><term>Parasite</term><term>Phylogenèse</term><term>Réaction chaîne polymérase</term><term>Systématique</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The hard tick Ixodes ricinus (Ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial bacterium, which has recently been named Midichloria mitochondrii. The objectives of this work were (i) to screen ixodid ticks for Midichloria-related bacteria and (ii) to determine whether these bacteria exploit the intra-mitochondrial niche in other tick species. Our main goal was to discover further models of this peculiar form of symbiosis. We have thus performed a PCR screening for Midichloria-related bacteria in samples of ixodid ticks collected in Italy, North America and Iceland. A total of 7 newly examined species from 5 genera were found positive for bacteria closely related to M. mitochondrii. Samples of the tick species Rhipicephalus bursa, found positive in the PCR screening, were analysed with transmission electron microscopy, which revealed the presence of bacteria both in the cytoplasm and in the mitochondria of the oocytes. There is thus evidence that bacteria invade mitochondria in at least 2 tick species. Phylogenetic analysis on the bacterial 16S rRNA gene sequences generated from positive specimens revealed that the bacteria form a monophyletic group within the order Rickettsiales. The phylogeny of Midichloria symbionts and related bacteria does not appear completely congruent with the phylogeny of the hosts.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>Italie</li><li>États-Unis</li></country><region><li>Colorado</li><li>Géorgie (États-Unis)</li><li>Languedoc-Roussillon</li><li>Nouvelle-Galles du Sud</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li><li>Sydney</li></settlement><orgName><li>Université de Sydney</li></orgName></list><tree><country name="Italie"><noRegion><name sortKey="Epis, S" sort="Epis, S" uniqKey="Epis S" first="S." last="Epis">S. Epis</name></noRegion><name sortKey="Bandi, C" sort="Bandi, C" uniqKey="Bandi C" first="C." last="Bandi">C. Bandi</name><name sortKey="Bandi, C" sort="Bandi, C" uniqKey="Bandi C" first="C." last="Bandi">C. Bandi</name><name sortKey="Clementi, E" sort="Clementi, E" uniqKey="Clementi E" first="E." last="Clementi">E. Clementi</name><name sortKey="Genchi, M" sort="Genchi, M" uniqKey="Genchi M" first="M." last="Genchi">M. Genchi</name><name sortKey="Magnino, S" sort="Magnino, S" uniqKey="Magnino S" first="S." last="Magnino">S. Magnino</name><name sortKey="Rinaldi, L" sort="Rinaldi, L" uniqKey="Rinaldi L" first="L." last="Rinaldi">L. Rinaldi</name><name sortKey="Sacchi, L" sort="Sacchi, L" uniqKey="Sacchi L" first="L." last="Sacchi">L. Sacchi</name><name sortKey="Sassera, D" sort="Sassera, D" uniqKey="Sassera D" first="D." last="Sassera">D. Sassera</name><name sortKey="Torina, A" sort="Torina, A" uniqKey="Torina A" first="A." last="Torina">A. Torina</name></country><country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Beninati, T" sort="Beninati, T" uniqKey="Beninati T" first="T." last="Beninati">T. Beninati</name></region><name sortKey="Beninati, T" sort="Beninati, T" uniqKey="Beninati T" first="T." last="Beninati">T. Beninati</name><name sortKey="Lo, N" sort="Lo, N" uniqKey="Lo N" first="N." last="Lo">N. Lo</name></country><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Beati, L" sort="Beati, L" uniqKey="Beati L" first="L." last="Beati">L. Beati</name></region><name sortKey="Piesman, J" sort="Piesman, J" uniqKey="Piesman J" first="J." last="Piesman">J. Piesman</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Mccoy, K D" sort="Mccoy, K D" uniqKey="Mccoy K" first="K. D." last="Mccoy">K. D. Mccoy</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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